Use conjugating in a sentence - Example Sentences for conjugating
Conjugating threads on the substratum by degrees elaborate zygospores, but these, contrary to the mode in other species, are surrounded by curious branched processes which emanate from the arcuate cells on either side of the newly-developed zygospore.
These cells become afterwards cylindrical by pressure, the protoplasm is aggregated into a mass, the double membrane at the point of first contact is absorbed, and the two confluent masses of protoplasm form a zygospore invested with a tubercular coat and enveloped by the primary wall of the two conjugating cells.
-Tilletia caries with conjugating cells.
At first, the lady met my preposition with declension, but in a short time we were conjugating magnificently, even recklessly.
SCF complexes share homologues of the core components cullin 1 (CUL1), SKP1, and the RING domain protein HRT1/RBX1/ROC1, which associate with different F-box proteins and ubiquitin-conjugating enzymes [ 1].
As Pcu3p-associated ubiquitin ligase activity was not described before, we first needed to determine which of the fourteen different ubiquitin-conjugating enzymes (UBCs) present in the fission yeast genome associate with the RING domain protein Pip1p, an essential cofactor of cullin ubiquitin ligases [ 7, 28, 29, 30].
For example, binding by CSN could protect Pcu3p from the Ned8p-conjugating enzyme Ubc12p, thus maintaining a fraction of Pcu3p in a deneddylated state.
Abbreviations CSN: COP9/signalosome GST: Glutathione S-transferase HA: hemagglutinin SCF: SKP1/Cullin/F-box protein complex UBC: Ubiquitin-conjugating enzyme c:\users\ab101345\desktop\data\written_1\technical\biomed\1471-2091-2-9.
The ubiquitylation reaction involves a minimum of three enzymes: An E1, which mediates the ATP-dependent activation of ubiquitin, and an E2, or ubiquitin conjugating enzyme (UBC), which, together with an E3 ubiquitin ligase, transfers ubiquitin to the target protein.
Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] .
In addition, we required an ubiquitin activating enzyme (E1) and an ubiquitin conjugating enzyme (UBC).
Abbreviations SCF: SKP1/CUL1/F-box protein UBC: ubiquitin-conjugating enzyme HA: hemagglutinin NLS: nuclear localization signal NES: nuclear export signal Authors' contributions All experiments were performed by VS.
The ubiquitin transfer reaction involves three enzymes: E1, which mediates the ATP-dependent activation of ubiquitin, and E2, or ubiquitin conjugating enzyme (UBC), which, together with an E3 ubiquitin ligase, transfers ubiquitin to the target protein.
CUL1, in turn binds to SKP1, and together with HRT1, mediates the interaction with the ubiquitin-conjugating enzyme CDC34/UBC3.
Abbreviations SCF = SKP1/Cullin/F-box protein complex UBC = Ubiquitin conjugating enzyme MIB = Mibolerone (7?
-17?
-dimethyl-19-nortestosterone) SKP2 = S phase kinase associated protein 2 CDK2 = cyclin-dependent kinase 2 Authors' contributions All experiments reported in this study were performed by L-F.
Based on reports that overexpression of chaperones [ 59 62 ] or a dominant-negative form of the ubiquitin-conjugating enzyme [ 63 ] can suppress the formation of poly-Gln protein aggregates, Kaytor and Warren [ 58 ] proposed a three-stage model wherein (i) molecular chaperones initially associate with the misfolded protein, (ii) failure to diminish the pool of misfolded protein leads to aggregation and ubiquitination and (iii) inefficient degradation of the aggregate by the proteasome is manifested by the accumulation of inclusion bodies.
COP10, which has been shown to interact with AtCOP1 in yeast two-hybrid experiment, encodes a ubiquitin conjugating enzyme (E2) variant [ 44 ] .
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