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Some difficulty is often experienced at first in finding the hanging drop, and if the first attempt is unsuccessful, the student must not on any account, whilst still applying his eye to the ocular, rack the body tube down (for by so doing there is every likelihood of the[Pg 87] front lens of the objective being forced through the cover-glass, and not only spoiling the specimen, but also contaminating the objective); but, on the contrary, withdraw his eye, rack the tube up, and commence again from step 2.

To cultivate organisms, thus isolated, apart from other associated or contaminating bacteria-i.

The blood from the hyperimmunes is defibrinated and a preservative added, and after it has been tested for potency and freedom from contaminating organisms, it is ready for use.

The tone of the stomach is destroyed, and a crude unassimilated chyle is absorbed by the lacteals, and carried into the blood, contaminating its whole mass.

As to corporations, parishes, vestry-boards, and similar gatherings of jolter-headed clods who assemble to exchange such speeches that, by heaven, they ought to be worked in quicksilver mines for the short remainder of their miserable existence, if it were only to prevent their detestable English from contaminating a language spoken in the presence of the sun-as to those fellows, who meanly take advantage of the ardour of gentlemen in the pursuit of knowledge to recompense the inestimable services of the best years of their lives, their long study, and their expensive education with pittances too small for the acceptance of clerks, I would have the necks of every one of them wrung and their skulls arranged in Surgeons' Hall for the contemplation of the whole profession in order that its younger members might understand from actual measurement, in early life, HOW thick skulls may become!

" The servant shrinks into a corner, into a corner of that hideous archway, with its deadly stains contaminating her dress; and putting out her two hands and passionately telling him to keep away from her, for he is loathsome to her, so remains for some moments.

While we may deplore that sex has become the dominating factor in many young people's lives, the goal should be to expand and emphasize the nonsexual means of personal expression ("liberation") available to them, not to return to the repressive and contaminating moral hypocrisies of a previous age.

Unfortunately, the practices of military and airline staff are contaminating the language of the greater public.

This catenation activity observed with topoisomerase I and Top67 was unlikely to be due to contaminating topoisomerase III activity since it was not observed with the ZD domain purified under almost identical procedures and a site-directed mutant with substitution of the active site Tyr319 by phenylalanine also did not exhibit this activity (Figure 6a).

Protein-free plasmid DNA was purified using Qiagen maxiprep columns (San Diego, CA) and contained predominantly supercoiled plasmid with no contaminating RNA or low molecular weight contaminants.

Our experiments in a reconstituted transcription system lacking contaminating AP-1 proteins and using DNA templates with high affinity NFAT sites demonstrate that NFATp can function as a transcriptional activator on its own.

The cell suspension was brought to 10 ml with cold RPMI media, and the desired T cell subset was obtained by magnetic removal of the contaminating cells.

Post extraction samples were treated with RNAse A at 37°C for 15 minutes to remove contaminating RNA.

Primers used were based upon previously published mouse primer sets [ 27 28 ] , or designed using MacVector from cDNA sequences between exons, avoiding the amplification of potentially contaminating genomic DNA in the total RNA sample (Table 3).

Due to the potential of contaminating the tumor tissue sample with normal stroma, allele loss in informative patients was defined as a >50% decrease in the ratio of the polymorphic band intensities in the tumor tissue versus that in the surrounding normal stromal tissue, this was quantified using a densitometer.

Primers and probes were designed from nonconserved sequences of the genes (allowing for isoform specificity), spanning sites where two exons join (splice sites) when such sites are known (preventing recognition of the assay to any potential contaminating genomic DNA).

Contaminating red cells were then lysed in distilled water, and the sample was applied to an LS separation column with a magnetic monocyte isolation kit in accordance with the manufacturer's instructions (Miltenyi Biotec, Auburn, CA).

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The word contaminating