Use conjugates in a sentence - Example Sentences for conjugates
24 cells increased by 20 to 31% within 2 h of incubating the cells in medium containing 100 µM of glycine conjugates of cholic acid, chenodeoxycholic acid, and deoxycholic acid (GCA, GCDCA, GDCA, respectively) (Figure 4A).
recently demonstrated that partially purified CSN promotes the cleavage of Pcu1p-Ned8p conjugates in vitro [ 10], but it remained unclear whether the enzymatic activity is contained within one of the CSN subunits or a tightly associated peptidase.
All peptidases known to cleave ubiquitin- or SUMO-protein conjugates are cysteine-based proteases (reviewed in ref.
Next, the Tyramide Signal Amplification (TSA™) was then used to amplify the Cy-3 and Cy-5 signals using antibody-enzyme conjugates, ?
-DNP-Horseradish peroxidase (HRP) and ?
-Streptavidin-HRP with Tyramide linked Cy-3 and Cy-5.
The membrane was then incubated with anti-rabbit IgG peroxidase conjugates, and proteins were visualized using the chemiluminescence method (Luminol reagent, Santa Cruz Biotechnology) and Kodak BioMax light film (Fisher Scientific).
Helix Pomatia enzyme was purified with activated charcoal and used for enzymatic hydrolysis of the phytoestrogen conjugates.
This results in the systemic accumulation of D-galatosyl conjugates, particularly globotriosylceramide (Gb3) [ 3 4 ] .
For such adenovirus-microbead conjugates, great enhancement in transduction efficiency was seen with several different cell lines, including COLO205, the infection efficiency of which was not notably enhanced by adenoviral vectors immobilized on streptavidin-coated wells in the present study.
The sizes of adenovirus-microbead conjugates are smaller than those of target cells.
Thus, the entire adenovirus-microbead conjugates can be taken up by cells.
Such internalization events appear to contribute substantially to the infectivity enhancement by virus-microbead conjugates.
This may be one possible explanation of why infectivity enhancement was seen with adenovirus-microbead conjugates and was not with adenoviral vectors immobilized on streptavidin-coated wells, except for one cell line (C6).
This study provides an extensive characterization of the new SP derivatives that will provide a basis for future studies involving the fluorescent conjugates.
In order to observe the direct-labeling of the SP conjugates to the SPR, CHO cells expressing the rat SPR (rSPR), were incubated with the SP analogs (100 nM) for 2 hrs at 4°C, to prevent internalization of the receptor, rinsed and fixed.
SP receptor binding An important determinant of the usefulness of the SP conjugates is their ability to bind to and activate the SPR.
Statistical analysis also demonstrated that the competition binding curves generated by the SP conjugates were significantly different than the unlabeled SP binding curve (p < 0.
Further analysis showed that, with the exception of the binding curves produced by tetramethylrhodamine-SP and Oregon Green 488-SP, a significant difference was seen between the binding curves of the different SP conjugates (p < 0.
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The word conjugates
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